DNA

Part:BBa_K3171177:Design

Designed by: Yashika Venkatesh   Group: iGEM19_Groningen   (2019-10-19)


HisD 3000bp Homologous part 1


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal XbaI site found at 689
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1472
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal XbaI site found at 689
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal XbaI site found at 689
    Illegal AgeI site found at 1885
    Illegal AgeI site found at 2788
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 807


Design Notes

Specific gRNA targets were selected for knockout of the HisD gene.


Source

HisD Homologous sequence

References